“Cell culture” refers to the in vitro proliferation of cells. For many years, efforts have been
mainly focused on optimizing culture conditions, including selection of appropriate culture medium, in order to achieve rapid cell growth. However, not enough focus has been on the cell culture environment itself. The conventional CO2 incubator only provides minimum requirements for keeping cells alive in a culture environment where the pH and temperature should be held at 7.4 and 37°C, respectively, and sub-saturated.