Given below are a few of the essential steps to remember during cell culture. Some of these are mandatory. Many of them are common sense and apply to all laboratory areas. Some of them are specific to cell culture.
Only handle one cell culture at a time. This common-sense approach will reduce the possibility of cross contamination. It will also reduce the spread of bacteria and mycoplasma due to the generation of aerosols across opened media bottles and flasks in the cabinet.
Test cells for the presence of mycoplasma on a regular basis.
Examine cultures and media daily for evidence of gross bacterial or fungal contamination. This includes media that has been purchased commercially.
Maintain separate bottles of media for each cell line in culture.
Quality control all media and reagents prior to use.